Journal: Neurotoxicity research

Article Title: Effects of Chronic Voluntary Alcohol Drinking on Thiamine Concentrations, Endoplasmic Reticulum Stress and Oxidative Stress in the Brain of Crossed High Alcohol Preferring Mice

doi: 10.1007/s12640-019-00032-y

Figure Lengend Snippet: Effect of chronic alcohol exposure on the expression of thiamine transporters in the brain and liver of cHAP mice. Male cHAP mice voluntarily drank alcohol for 7 months as described in the Materials and Methods. A. The expression of SLC19A2, SLC19A3 and OCT1 in the cerebrum was examined by immunoblotting. The expression of actin served as a loading control. The relative amounts of SLC19A2, SLC19A3 and OCT1 were quantified and normalized to the expression of actin. The experiment was replicated three times, and the results were expressed as the mean ± SEM, n = 4 or 5 for each group. *p< 0.05 denote statistically significant difference from the control group. B. The relative expression of SLC19A2, SLC19A3 and OCT1 in the liver was examined by immunoblotting, and quantified and normalized to the expression of actin. Each data point was the mean ± SEM of three independent experiments, n = 4 or 5 for each group. *p< 0.05 denotes a statistically significant difference from the control group.

Article Snippet: Anti-SLC19A3 antibody was purchased from Proteintech (Rosemont, IL).

Techniques: Expressing, Western Blot, Control

Journal: BMC Biotechnology

Article Title: Application of surface plasmon resonance imaging to monitoring G protein-coupled receptor signaling and its modulation in a heterologous expression system

doi: 10.1186/s12896-016-0266-9

Figure Lengend Snippet: PKC activation results in an increase in R P/S . ( a and b ) TPA (100 nM) but not the control vehicle (DMSO) alone increased R P/S in non-transfected HEK293T cells. In this and following figures, the test agent-containging saline was dropped into the measurement chamber after a 5-min basal measurement; the test agent continued to be present in the chamber onward. a Sample images of the reflection of the P-polarized light before and after a 25-min application of the labeled agent. b Mean time-plots of R P/S . Arrow, onset of test agent application. Error bars, ±SEM. ***, p < 0.0001 at 25 min of the test agent application onset (van der Waerden rank sum test)

Article Snippet: TPA (4174S, Cell Signaling Technology, Danver, MA, USA) was dissolved in DMSO (13407-45, Nacalai Tesque) to a concentration of 2 μM and kept at -50 °C until use.

Techniques: Activation Assay, Control, Transfection, Saline, Labeling